Specificity and kinetics of triose phosphate isomerase from chicken muscle
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چکیده
منابع مشابه
Folding and association of triose phosphate isomerase from rabbit muscle.
The enzymatic activity and quaternary structure of rabbit muscle triose phosphate isomerase remains unchanged in the concentration range from 2 micrograms/ml to 2 ng/ml. In this concentration range the enzyme can be reactivated after dissociation and denaturation in 6.5 M guanidine hydrochloride. Removal of the denaturant by dilution and separation of inactive wrong aggregates (5-20%) lead back...
متن کاملThe active centre of triose phosphate isomerase.
The molecular weight and amino acid composition of triose phosphate isomerase have been determined. The molecular weight (43000) is lower and the molecular activity (500000) higher than those of most other glycolytic enzymes. Reaction with iodoacetate (studied with radioactive reagent) takes place in two phases: in the first phase, at pH6.3, cysteine and methionine groups react and enzymic acti...
متن کاملErythrocyte lipids in triose-phosphate isomerase deficiency.
Marked hypoalphalipoproteinemia was found together with relatively low serum cholesterol, triacylglycerol, and LDL levels in a triose-phosphate isomerase (TPI; D-glyceraldehyde-3-phosphate ketol-isomerase, EC 5.3.1.1)-deficient Hungarian family, especially in the two compound-heterozygote brothers. Apart from a slight increase in palmitic and stearic acids together with a slight decrease in ole...
متن کاملInactivation of muscle triose phosphate isomerase by D- and L-glycidol phosphate.
Dand L-glycidol phosphate have been synthesized in order to investigate the stereospecificity of the inactivation of triose phosphate isomerase by glycidol-P. Both enantiomers inactivate the enzyme. Similar concentrations for half-maximal rates of inactivation are found, but maximum Vinact is IO-fold larger for D-glycidol-P than for L-glycidol-P. The same glutamate residue of the protein is est...
متن کاملDegradation of functional triose phosphate isomerase protein underlies sugarkill pathology.
Triose phosphate isomerase (TPI) deficiency glycolytic enzymopathy is a progressive neurodegenerative condition that remains poorly understood. The disease is caused exclusively by specific missense mutations affecting the TPI protein and clinically features hemolytic anemia, adult-onset neurological impairment, degeneration, and reduced longevity. TPI has a well-characterized role in glycolysi...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1972
ISSN: 0306-3283
DOI: 10.1042/bj1290301